TGF-β1 secreted by epiphyseal plate chondrocytes in serum-free medium

Objective To observes the growth of epiphyseal plate chondrocytes in serum-free medium and detect the TGF-β1 secreted from epiphyseal plate chondrocyes by ELISA.Methods Epiphyseal plate chondrocytes were isolated from the epiphyseal tissue of New Zealand rabbits at the age of three weeks.Growth of epiphseal plate chondrocytes in serum-free medium was observed following the CCK-8 growth curve.The TGF-β1 secreted by epiphseal plate chondrocytes was detected by ELISA.Results The epiphseal plate chondrocyes grew quite well and secreted the extracellular matrix.AO/PI fluorescence staining showed bright green surviving cells with a small number of epiphseal plate chondrocyes red-stained around.The morphology of growth curves of the epiphseal plate chondrocyes in DMEM and serum-free medium was similar.The proliferation of epiphseal plate chondrocytes was slow on the first 2 days,then increased gradually,and entered the platform period on the 6-7 days.The epiphseal plate chondrocytes began to release the TGF-β1 on the first day,then increased to release it gradually,and the concentration of TGF-β1 reached 167.2pg/ml on the sixth day.Conclusion The epiphseal plate chondrocytes can grow well in serum-free medium and secrete TGF-β.