Method for SARS virus antibodies level determination and its application

Objective:To establish methods for testing the SARS IgG or IgM antibodies level in patients.Methods:① Based on the diagnosis kits for SARS-coronavirus IgG or IgM antibodies qualitative analysis，we obtained the concentration and absorbency curve of SARS-coronavirus IgG or IgM antibodies and the data fitted equations were derived through serial dilution of the selected standardized serums.The fitted equations were applicable for calculating the SARS-coronavirus IgG or IgM antibodies level in serum.② SARS-coronavirus IgG or IgM antibodies level of eighty convalescent in-patients with SARS were analyzed by these methods.Results:① Favorable fitted equations were obtained the R square of which was more than 0.99.② Sixty one of eighty convalescent in-patients with SARS had IgG antibodies positive，and the relative level of IgG antibodies were 31.9± 40.0，ranging from 1.1 to 264.0 and its skewnessis 3.677.nineteen of eighty convalescent in-patients with SARS had IgM antibodies positive，and the relative level of IgM antibodies were 2.59±1.35，ranging from 1.1 to 5.5 and its skewness is 0. 927. Conclusion:① Based on the favorable sensitivity and specific of the kits，Excel can give the fitted equations which characterized the absorbency and the relative level of IgG or IgM antibodies in the serum.②The distribution of IgG antibodies relative level in the convalescences whose SARS virus antibodies were positive was skew and scatter.