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Xin Jun, Wang Xiaoxiong, Meng Shuli, Wang Xin, Feng Dongxiao, Li Chunhai. The Costruction of pZip IFN-γ——A eukaryotic expression vector for human interfe ron-γ geneJ. ACADEMIC JOURNAL OF CHINESE PLA MEDICAL SCHOOL, 1997, 18(1): 4-5.
Citation: Xin Jun, Wang Xiaoxiong, Meng Shuli, Wang Xin, Feng Dongxiao, Li Chunhai. The Costruction of pZip IFN-γ——A eukaryotic expression vector for human interfe ron-γ geneJ. ACADEMIC JOURNAL OF CHINESE PLA MEDICAL SCHOOL, 1997, 18(1): 4-5.
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The Costruction of pZip IFN-γ——A eukaryotic expression vector for human interfe ron-γ gene

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  • The aim of this study was designed to construct a retrovirus vector expvessing human Interferon-γ（IFN-γ）gene in eukaryotic cell. The human IFN-γ cDNA was isloated from pGEM 1 IFN-γ,then it was inserted into retrovirus vector pZip· Neo·SV（X）1. The results of enzymic hydrolysis comfirmed that the human IFN-γ cDNA was correctly cloned to pZip·Neo·SV（X）1. The success in construction of human IFN-γ expression vector makes it possible to carry out further research in gene therapy.
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