The application of real-time FQ-PCR technique in detection of HBV-DNA

Objective:To exqlore the value of HBV-DNA copies detected by real-time fluorescence quantitative PCR（FQ-PCR） in the diagnosis and therapy of HBV infection.Methods:523 patients’ serum HBV-DNA were detected by real-time FQ-PCR.The cutoff is 500 copies/ml.Their serological markers of HBV were detected by ELISA method.Both results of HBV-DNA and serological markers of HBV were compared.Results: In the 116 HbsAg（+）,HbeAg（+）,HbcAb（+） and ProS1-Ag（+） samples,HBV-DNA positive ratio and mean of copies are 98.7% and 1.87×10~8copies/ml.They are much higher than that of other groups（P＜0.05）.The HbsAg（-）,HbeAg（-）,ProS1-Ag（-） group’s positive ratio and copy number of HBV-DNA are much lower than that of HbsAg（+） and/or HbeAg（+）,and/or ProS1-Ag（+） group（P＜0.05）.Conclusion:FQ-PCR is a fast and accurate technique to quantitative the serum HBV-DNA.HBV-DNA can reflect HBV replication accurately.Combination the HBV-DNA and the serological markers of HBV would make the diagnosis and therapy of HBV infection more reasonable and efficient.