Method of cytoperservation APA microencapsulated 3T3 cells

Objective: To compare the effects of frozen stock solutions with different components and concentration on low temperature preservation of APA microencapsulated mouse fibroblast cells （APA-3T3）, and to explore the optimal condition of APA microencapsulated cells low temperature preservation. Methods: Capsulated 3T3 cells with Alginate-Polylysine-Alginate （APA） to get APA-3T3, and programmed reduced the temperature of APA-3T3 in frozen stock solutions with different concentration of DMSO and BSA, and then preserved in liquid nitrogen. Checked the form and permeability of APA-3T3 after rapid rewarming, and recorded the motility rate of cells with trypan blue dyeing. Results: Compared with other DMSO groups, the 10%DMSO group has the maximal motility rate, about（75.68±1.54）%, P＜0.01, and microcapsule undamaged rate, about （94.48± 0.90）%, P＜0.01; Compared with other BSA groups, the 4%BSA group has the maximal motility rate, about（73±1.26）%, P＜0.01, and microcapsule undamaged rate, about（85.1±0.82）%, P＜0.01; The frozen stock solutions with different components and concentration have no remarkable effect on the permeability of microcapsule. Conclusion: The frozen stock solution with 10%DMSO and 4%BSA can preserve the cells and permeability of microcapsules in the process of low temperature preservation.