The experimental study of radioimmunoguided surgery for lung neoplasm and the preliminary clinical practice of radio guided surgery for lung neoplasm

Objective: The tumors and the micrometastases of the mice model bearing pulmonary adenocacinoma were detected by means of hand-held gamma detecting probe (GDP)；the preliminary clinical practice of radio guided surgery (RGS) was underwent to detect the tumors and the micrometastases at the time of surgery.Methods: Using biotin-avidin pretargeting technique, the experimental study of RIGS on the mice bearing pulmonary adenocacinoma models was performed with antihuman lung adenocacinoma monoclonal antibody (LC-1).Using Peplomycin (PPM) or Methoxyisobutylisonitrile (MIBI) as tumor tracer，radioactivity of the specimens resected from the the patients were detected by means of GDP at time of surgery.Tumortonorma-l tissue (T/NT) count ratio were obtained by comparing the radioactivity of the tumor tissue with the normal lung tissue. Results: The T/NT ratio of the tumor and the metastatic lung of the mice were significantly high.Based on the application of GDP to identify the metastases of lung，the sensitivity、the specificity and the accuracy was 98%、96% and 97% respectively. Using GDP could accurately detect the tumors and the metastases of lymph node，the sensitivity、the specificity and the accuracy was 91%，88% and 90% respectively.Conclusions: The tumors and the micrometastases of the lung could be detected in vivo by GDP.PPM and MIBI as tumor tracer can be used in detecting tumor by means of GDP intraoperatively.