Lactobacillus’ sticky and depressant effect on uterine cervix cancer cells

Objective To explore lactobacill'u ssticky and depressant effect on uterine cervix cancer cells. Methods 1.Cervix uteri amotic epithelialis cells from 30 women were shake cultured with lactobacillus 1-3h,and the lactobacillus’ sticky index number on cervix uteri amotic epithelialis cells was observed through Gram's stain.2.Uterine cervix cancer cells（Hela cells） and lactobacillus were co-cultured 1-72h,and the lactobacillus’ sticky index number on cervix uteri cancer cells and cell activity changes at different times were observed. Results 1.Lactobacillus had good sticky effect on cervix uteri amotic epithelialis cells: There appeared sticky index number climax（viable lactobacillus 39.44±2.5;inactivation lactobacillus 85.1±2.9） 2h after the co-culture began.Inactivation lactobacillus had better sticky effect on cervix uteri amotic cellula epithelialis,and the sticky index number of inactivation lactobacillus was higher than that of viable lactobacillus 2-3h after the co-culture began（P＜0.01）.2.The sticky effect of lactobacillus on uterine cervix cancer cells increased gradually with the culture time.There appeared sticky climax（viable lactobacillus 25.69±3.1;inactivation lactobacillus 90.49±2.6） 12h after the co-culture began;but after that,the sticky index number decreased gradually.3.At the beginning of the co-culture,lactobacillus did not affect the activity of uterine cervix cancer cells.In viable lactobacillus,the activity of uterine cervix cancer cells began to decrease 12h after the co-culture began;and in inactivation lactobacillus,the activity of uterine cervix cancer cells began to decrease 24h after the co-culture began. Conclusions Lactobacillus could stick and protect cervix uteri cellula epithelialis and prevent paraplasm of cervix uteri cellula epithelialis.Lactobacillus could also stick and restrain uterine cervix cancer cells.Thus inactivation lactobacillus had better sticky and depressant effect on uterine cervix cells and uterine cervix cancer cells.