Expression of matrix metalloproteinase13 and its mRNA in carbon-tetrachoride-injured liver fibrosis and its reversal

Objective: To explore the expression of matrix metalloproteinase 13(MMP-13) and its mRNA in carbon-tetrachoride-injured liver fibrosis and its reversal. Methods: By immunohistochemical analysis and nucleic acid in situ hybridization(ISH) we observed the expression of MMP-13and its mRNA in specimens from liver of Wistar rats induced by carbon tetrachloride. Results: Expression of MMP 13and its mRNA was found mainly in mesenchymal cells,also in parts of hepatocytes.The levels of MMP-13expression were increased in the early time of liver fibrosis and decreased gradualy in the reversal of liver fibrosis. Conclusions:Mesenchymal cell is the main cellular origin of MMP13and its mRNA. MMP 13may play an important role in the liver fibrogenesis and its reversal.