SAGE pattern of LRP16 gene and its expression in normal blood and leukemic cells

Objective: To investigate the possible significance of LRP16during tumor development and progression and during hematopoietic maturation,bioinformatic and experimental method were employed to detect the expression state of LRP16in tumor development and different hematopoietic stages. Methods: Using CGAP/SAGEmap database provided by NCBI as experimental material,"Virtual Northern"program as tool,LRP16expression pattern was compared among multitumor tissues,cell lines and corresponding normal tissues.Sem-i quantified RT-PCR was adopted to compare the expression amount among normal blood cells and leukemic cells. Results: Analysis of SAGE pattern showed that LRP16gene was highly expressed in several human tumor cells than in their normal counterparts.LRP16was not detected in cord blood cells.The expression amount of LRP16gene in normal bone marrow and PBSC(peripheral blood stem cells)mobilized by G-CSF were significantly lower than in normal peripheral blood,primary leukemic cells and leukemic cell lines(P<0.0001)such as HL60,K562 and U937. Conclusion: These results suggested that LRP16have different expression level in different blood cells,and demonstrated that LRP16is related to AL and many kinds of human tumors. The expression difference between diagnostic and relapsed AML is valuable to be further investigated.