LRP15-gene-promoter methylation patterns in hematological diseases

Objective: To explore the methylation status of LRP15 gene in hematological diseases and its role in the tumorigenesis.Methods: Methylation patterns were detected in 9 healthy donors、 73 acute leukemia（AL） patients、 8 chronic myeloid leukemia（CML）patients、 2 chronic lymphocytic leukemia（CLL） patients and 12 patients with benign hematological diseases by methylation specific PCR（MSP）.COBRA（Combined bisulfite restriction analysis） and bisulfite sequence analysis confirmed LRP15 methylation in an adult with benign hematological diseases.Results: The frequency of LRP15 methylation was（71.23%）（52/73）in AL patients,0%（0/9） in healthy donors and 10%（1/10） in CL patients.The diffrence of LRP15 methylation frequency between AL patients and healthy donors was statistically significant （P=0.000）.The difference between AL patients and CL patients was also statistically significant.LRP15 methylation in 55.56%（5/9）adult patients with hematological benign diseases was detected for the first time.COBRA and bisulfite sequence analysis confirmed LRP15 methylation in an adult with benign hematological diseases. Conclusions: LRP15 methylation change is common abnormality in immature leukemia cells.LRP15 may be a putative tumor suppressor gene.It is possible that hypermethylation of LRP15 gene is related to the pathogenesis and development og hematological benign diseases.