Insulin-like growth factor-1 increased the calcium deposition of vascular calcification in vitro

Objective: Accumulated evidence suggested that vascular calcification share many characteristics with the minaralization of bone. IGF-1can enhance the minaralization of osteoblastic cell in bone and is also one of the important growth factors in blood vessel. So it is rational to study its role in the vascular calcification. Methods: β-glycerin-phosphate disodium salt was used to induce cultured bovine vessel smooth muscle cell to calcify which serves as a vascular calcifying model in vitro. 10^-9mol/L IGF-1were add to the culture media. After 10days culturing,the deposition of calcium,alkaline phosphatase activity and osteocalcin concentration was quantified. Results: After 10days of culture,10^-9mol/L IGF-1increased the calcium deposition of calcified BVSMC by 11. 82%(P>0. 05),this processes is time dependent. The cellular ALP activity was dramatically increased with the highest in the 8day with the greatest increase of 168. 7%. The osteocalcin concentration of cultured media syntheses by BVSMCs also elevated 58. 3%. Change the concentration of IGF-1 to 10^-8 mol/L and 10^-7 mol/L,the calcium deposition of BVSMCs increased by 22. 74% (P <0. 05) and 40. 7% (P <0. 01) respectively. Conclusion: IGF-1 increased calcified BVSMCs'calcium deposition. This may through the stimulating the cellular synthesis of ALP and osteocalcin which indicate that it promote the calcified BVSMCs'changing to osteoblastic cell.