Epitope analysis of interaction between FHL2 and Id proteins

Objective: To investigate the interaction between FHL₂ and Id proteins and its corresponding epitope. Methods: Interaction between FHL₂ and Id proteins and the corresponding epitope conferring this association were detected by GST pulldown assays. Results: FHL₂ directly interacted with all Id proteins.The second LIM domain containing FHL₂ or N terminal region in Id proteins was essential for the direct binding of FHL₂ to Id proteins. Conclusion: FHL₂ is a novel and broad interacting factor of the Id proteins.Through cooperating with Id proteins,FHL₂ may be involved in multiple biological effects and tumorigenesis mediated by Id proteins.