Analysis of different proteins in gall-stone and biliary cast patients using HD/MS

Objective To study the mechanism of biliary cast formation by analyzing different proteins in gall-stone and biliary cast patients. Methods Total protein solution was extracted from gall-stone and biliary cast samples and digested with enzyme. The proteins were identified using HD-MS. Results Of the 271 proteins identified from the total protein solution of all samples, 11 were found to be unique proteins of biliary cast. Conclusion The 11 identified unique proteins of biliary cast can provide a significant clue for studying the mechanism of biliary cast formation.