Determination of immuisolation effect of APA microcapsules in vitro

Objective: to understand immuisolation effect of Alginate polylysine alginate（APA) in vitro. Materials: Checking the diffusion through APA microcapsule membrane of IgG labeled with fluorescein by laser scan confocal microscope（LSCM). Assessing injury effect of NK cells to K562 or encapsulated K562. Assessing protection effect of APA microcapsule from mouse spleen lymphocyte proliferation stimulated by BCCs. Checking injury effect of IL-2 and TNF α to cells or microencapsulated cells. Result s: IgG can not go through into APA microcapsule.NK cells have cytotoxic effect to K562 cells and APA can protect K562 from attack of NK. APA can prevent mouse spleen lymphocyte proliferation stimulated by BCCs.IL-2 can pass through APA membrane and support existence of CTLL cells. TNF αcan pass through APA membrane and kill L929 cells. Conclusion: APA microcapsule can remarkablely prevent cell-mediated and antigen mediated immunologic reactions.