Changes of procoagulant activities in acute lung injury and effects of fructose-1,6-diphos-phate

Objective: To observe changes of procoagulant activities in pulmonary tissues of rabbits with endotoxin（ET）-induced acute lung injury（ALI） along with the effects of fructose-1,6-diphosphate（FDP）,and to study the possible antagonizing effects of FDP. Methods: Flap-eared white rabbits were randomly assigned to three groups: control group,injured group（treated by ET） and intervened group（treated by ET and FDP）.ALI was induced by the injection of ET at a time.During the experimental course,respiratory rates,heart rates,blood pressures,arterial blood gases and blood cell counts were measured at 0 h,（0.5 h）,2 h,4 h and 6 h respectively.After rabbits sacrificed at 6 h,the pulmonary contents of Thromboxane B₂(TXB₂) and 6-keto-prostaglandin F_1α(6-keto-PGF_1α) were measured,and the pathologic changes were also examined. Results: Compared with control group,injured group showed the typical characters of ALI,including respiratory rates enhancement,heart rates increase,blood pressure decrease and arterial blood oxygen pressure drop.The increase of TXB₂/6-keto-PGF_1α ratio in lung tissues were also seen in injured group.The pathologic injuries of lung tissues were much more severe in injured group. Conditions in intervened group were better than those in injured group. Conclusion: Enhancement of procoagulant activities in pulmonary tissues is important in pathogenesis of ET-induced ALI.FDP can inhibit procoagulant activities in pulmonary tissues,and protect rabbits from ET-induced ALI to some extent.