The effect of Rubiaquinone on viability of cadaver cryopreservated skin

Objective: To discuss the effect of Rubiaquinone on the cryopreservation of skin as a additive to cryoprotectent.Methods: Low speed cooling method and vitrification were adopted as the method of skin cryopreservation. Three group which contain none, 0.5μg/ml, 1μg/ml Ruibiaquinone were created for each method. After preserved at liquid nitrogen, the viability of small sheet of guinea pig and cadaver skin were assessed by oxygen consumption and SDH. Then average viability is also calculated.Results: Our results showed that Rubiaquinone had no effect on whatever guinea pig and cadaver skin with low speed cooling or vitrification.Conclusion: Our data indicated that the peroxide injury in cyropreservation was different from that at 4℃. Further research should be carried out for understanding its inherent machanism.