Changes of the visceral vascular permeability dring early burn stage and their relationship with pathological alterations in rats

Objective:To investigate the changes in the visceral vascular permeability in rats in early burn stage and its relationship with pathologic alteration.Methods:Using a rat model with 35% TBSA fullthickness burn, we have investigated the changes in water and protein content in five organs （heart, lung, intestine, liver and kidney） before and afterburn and their relationship with morphological alterations. Results:（1）The water content significantly increased in all organs from postburn hour 3 （PBh3） and reach their peaks within PBh48. The changes in heart and lung were relatively earlier whreras the changes in lung and kidney were more significant.（2）There existed a decrease in protein content in each organ from PBh8 to PBh72, especially in heart, liver and kidney. （3）There was a significant pathological change around PBh8 which menifested as a denature or edema of visceral vascular endothelial cells and a local necrosis of some stromal cells.（4）Immunohistological staining showed a significant enhancement in positive reaction of ET-1 in visceral stromal cells at PBh8. Conclusion:In. early burn stage in rats, there exists a pathological changes with a character of denature of vascular endothelial cells and elevation of vascular permeability.