The study of osteogenous active on the surface of carbonated hydroxyapatite

Objective: To investigate the surface osteogenous activity of carbonated hydroxyapatite （CHA）. Methods: The cellar adhesion, proliferation, alkaline phosphatase （ALP） activity and Ⅰ collagen（COLI） mRNA expression were observed by culturing MC3T3-E1 cells on the surface of CHA, and bone cement polymethylmethacrylate（PMMA） disk being control group.Rusults:The cellar adhesion, proliferation, ALP activity and COLI mRNA expression on the surface of CHA were obviously higher than PMMA. Conclusion: These results highlight the fact that CHA cement has excellent biocompatibility and osteogenous activity, which indicated that CHA is likely to be an optimal biomaterial for bone defect repairing.