Determination of ATP in the rat heart with ischemia reperfusion by ion-pair reversed-phase HPLC

Objective: To study the change alteration of ATP in the rat heart with ischemia reperfusion injury by lon-pair reversed-phase HPLC. Methods: Ion-pair reversed-phase HPLC methods was developed for determination of ATP.The solvent consist of 0.1 mmol·L^-1 KH₂PO₄;8 mmol·L^-1 tetrabutylammonium hydrogen.The separation was monitored at 259nm.（Results: The） calibration curve showed good linearity（r=0.998）.The coefficients of variation（C.V） were estimated from（repeated） determinations of standard ATP.The intraassay C.V varied from 1.91% to 5.78%.The recovery was between 97.25% and 100.05%. Conclusions: This methods is precise,it can be used for determination of ATP in the rat heart.