Study of total flavonoids of Astragalus’s biology action on chemical ingredient

Objective: To study the main chemical structures and its proportion for understand the mechanism action of total flavonoids of Astragalus（TFA）. Methods: The chemical fractions of TFA have been separated by different type column.The chemical structures have been identified. Meanwhile,the proportions of all chemicals from TFA have been calculated.（Results: Six） single chemical from TFA have been identified.There was β-sitosterol（1）,formononetin（2）,calycosin（3）,daucosterol（4）,formononetin-7-O-β-D-glucopyranoside（5） and calycosin-7-O-β-D-glucopyranoside（6）.The proportion of the six chemicals was 23∶11∶12∶3∶22∶29. Conclusion: TFA is a mixture which contains six main chemicals.The potential biological activity actions were related with the special proportions of the six chemicals.