The establishment and application of the method to respiratory syncytial virus polymerase chain reaction sequencing strategy

Objective: To search the method of respiratory syncytial virus cDNA（RSV cDNA） sequencing strategy. Methods: The polymerase chain reaction （PCR） was combined with the sequencing strategy by using γ³²P-ATP labeled T 7 Promoter as sequencing primer and the thermostable DNA polymerose as directed sequencing strategy.Results: The sequencing ladder was clear and readable. Although NS、N genes of RSV were rather stable, there were still exchanging mutations.Conclusion: The PCR sequencing strategy is a simple and reliable method for detecting RSV and its variability in patients with recurrent RSV infections.