The proliferation of neural stem cells in hemi-parkinsonian rats

Objective: To investigate the proliferation of neural stem cells in the rat model of Parkinsons disease .Methods: Rat model of Parkinsons disease was made by injecting the 6-hydroxydopaminin in the striatum.The rats were killed at different time points,and their brain were taken out.Before they were killed,the rats were injected Brdu into their abdomen.The dynamic expression of Nestin and Brdu were determined by immunocytochemistry,Brdu labeling method was used to mark the cells of proliferation .Nestin expression was used to identify neuroprogenitor cells.And they both used to mark the diving neural stem cells.Rsults: Campared with the controls,the number of Nestin-positive cells,Brdu-positive cells,and Brdu-Nestin-positive cells increased strikingly at 3,5,7days,in the hippocampus, then declined at 14 days and almost reached the normal 28 days after.（Conclusion: ）The rat model of Parkinsons disease by direct injection of 6-OHDA in the striatum stimulates the proliferation of inherent neural stem cells.