Effect of amplified autologous NK and T cells infusion from peripheral blood lymphocyte on tumor immunotherapy

Objective: To study the change of blood lymphocyte phenotype after amplified autologous NK and T cells infusion from peripheral blood lymphocyte in 22 patients with cancer.Methods: Mixture（named NKTm） of NK and T lymphocytes cells amplified from peripheral blood in malignant tumor patients was infused with 2 weeks interval.Before infusion and 2 weeks after infusion,the lymphocyte phenotype was detected by flow cytometry included CD3⁺、 CD3⁺CD4⁺、 CD3⁺CD8⁺、 CD3^-CD16^-CD56⁺、 CD3^-CD16⁺CD56^-、 CD3^-CD16⁺CD56⁺、 CD3⁺CD16^-CD56⁺、 CD3⁺CD16⁺CD56^-、 CD3⁺CD16⁺CD56⁺.Results: Two weeks after infusion,the phenotype of CD3⁺、 CD3⁺CD8⁺、 CD3⁺CD4⁺ cells did not significantly change,while the NK like cells（marked with CD16⁺/CD56⁺） statistically incresesd（P＜0.01）.The results did not changed after multiple doses.No side-effects were found.Conclusion: Amplified autologous NK and T Cells infusion from peripheral blood lymphocyte may increase the antitumor immune without any side-effects.