Study of apoptosis of salivary adenoid cystic carcinoma induced by TNF-α and paclitaxel in vitro

Objective: To explore the apoptosis mechanism of salivary adenoid cystic carcinoma cells（SACC-83） induced by recombinant human tumor necrosis factor α（rhTNF-α） and paclitaxel.Methods: The SACC-83 cells and primary culture of L929 cells were divided into nine groups of different medicine concentration and exposed for 72h.The inhibition ratio of cell proliferation was detected with ³H-TDR.The percentage of apoptotic cells and cell cycle distribution were detected by the flow cytometer.The cell morphological change of apoptosis was observed.Results: The inhibition ratio of cell proliferation in associated drugs group was significant higher than that of either single drug group（P＜0.05）.The inhibition ratio of cell proliferation of every group of L929 cell was significant lower than that of SACC-83 cell（P＜0.05）.Conclusion: TNF-α associated with paclitaxel is synergetic to inhibit SACC-83 cells and induce apoptosis.