Detection of anti-HCV antibody with different methods

Objective To investigate the impact of different methods and reagents on detection of anti-HCV antibody in blood donors. Methods Anti-HCV antibody in blood donors was detected by enzyme-linked immunosorbent assay（ELISA）using different reagents,and positive sera were examined by Western blotting confirmatory test. Results Western blotting confirmatory test showed a lower positive anti-HCV rate than ELISA（P＜0.05）. Conclusion Positive HCV antibody detected by ELISA should be confirmed by quantifying fluorescence in HCV RNA RT-PCR.