Angiotensin Ⅱ AT1 receptor antagonist reduces adipose tissue in rat

Objective: To investigate the effects of Angiotensin Ⅱ AT1 receptor antagonist-Candesartan on the morphology and function of rat adipocytes. Methods: 20 male Wistar Kyoto rats were randomly divided into vehicle and Candesartan treated groups.The dosage of Candesartan was 10 mg·kg^-1·d^-1.Food intake and bodyweight were recorded each week. Rats were sacrificed by decapitation after 17 weeks.Epididymal and peri-renal fat were collected and weighted.Adipocytes were isolated from epididymal adipose tissue with collagenase.Diameter of adipocyte was evaluated.Triglyceride was isolated from epididymal adipose tissue and determined.Blood chemistry,insulin,Leptin and adiponectin were assayed.mRNA expression of leptin,adiponectin and preadipocyte factor-1 of epididymal adipose tissue were detected with RT-PCR. Results: In Candesartan treated group,bodyweight and content of adipose tissue were significantly decreased;Adipocyte was shrunk and cellularity was increased.Total number of adipocytes was unchanged in two groups.Blood glucose and insulin level were no difference in two groups.Plasma Leptin level as well as Leptin mRNA expression in adipocyte tissue were lower, plasma adiponectin level as well as adiponectin mRNA expression were higher in Candesartan treated group.Preadipocyte factor-1mRNA expression was no significant difference in two groups. Conclusions: Angiotensin Ⅱ AT1 receptor antagonist decreases adipose tissue by lower the volume of adipocyte and enhance synthesis as well as releasing of adiponectin in rats.