Effect of Nfr3 on liver cancer SMMC7721 cell line in vitro

Objective To observe the effect of Nfr3 on liver cancer SMMC7721 cell line in vitro. Methods Plasmids were constructed by inserting the candidate gene and transfected into liver cancer SMMC7721.Cell cycle and apoptosis of liver cancer SMMC7721 cell line in vitro were detected by flow cytometry（FCM）,and the subcellular localization of this candidate gene was observed under a fluorescence microscope. Results The expression vector pEGFP-N1-Nrf3 was constructed.FCM showed that the number of SMMC7721 cells in G2/M+S and G0/G1 was significantly decreased and increased,respectively,under the influence of Nrf3.DNA synthesis and mitosis of SMMC7721 were restrained by Nrf3.FCM did not show the peak of deuto-G1（the peak of apoptosis）. Conclusion As a tumor-suppressing gene,Nrf3 can inhibit in vitro cell proliferation of Civer cancer cells but has no effect on their apoptosis.