Association of Gln223Arg polymorphism in leptin receptor gene with metabolic abnormalities in IGT and type 2 diabetes

Objective: To investigate the association of Gln223Arg polymorphism in leptin receptor gene with metabolic abnormalities in IGT and type 2 diabetes.Methods: The genotypes of Gln223Arg polymorphism in leptin receptor gene were determined by polymerase chain reaction fragment length polymorphisms （PCR RFLP） assay in 198 unrelated subjects of Beijing population of different glucose tolerance（including 104 subjects with normal glucose tolerance,58with impaired glucose tolerance and 36with type 2 diabetes） We also analyzed the clinical data.Results: No significant difference of genotype frequency of Gln223Arg polymorphismin leptin receptor gene was observed among NGT,IGT and DM Serum leptin level was not different between genotypes "A" allele was associated with increased cholesterol（7 26±1 95）vs（5 31±1 31）mmol/L, P=0.04, blood pressure and insulin resistance （HOMA） in male IGT.Conclusion: Gln223Arg polymorphism in leptin receptor gene is associated with disordered lipid in IGT