Amplifi cation of recombinant adenovirus in HEK293T cells

Objective To determine if recombinant adenovirus can be amplified in HEK293T cells. Methods After HEK293T cells were infected with a preservation solution of adenovirus,its effect on cytopathy was observed.Infectivity of amplified adenovirus was assayed with 50% of tissue-culture infective dose.Infectivity of adenovirus was confirmed with infected myocardial cells and fibroblasts in vitro. Results HEK293T cells promoted generation of recombinant adenovirus with green fluorescent protein.The titer of adenoviruses could reach 5×106PFU/mL.Adenoviral particles could infect myocardial cells and fibroblasts in vitro. Conclusion Recombinant adenovirus with green fluorescent protein can be amplified in HEK293T cells.