Rapid detection of the toxigenic vibrio cholerae and genotyping

Objective: To study a method quickly to indentify toxigenic vibrio cholerae and genotyping for meeting the requirement of clinical diagnosis and test of foods.Methods: Polymerase chain reaction （PCR）was used to detect cholera toxin（CTX） A2 B subunit gene of a target and randomly amplified polymorphic DNA （RAPD） to play a role of genotype.Results: 3 isolates of V cholerae strains O139, 3 isolates O1 biotype El Tor, 2 isolates O1 biotype classical containedthe 566bp segment of genomic sequences of ctx A2 B which were homologous （97 1%～98 9%）confirmed by nuceotide sequence analysis The isolates of V cholerae were divided into 2 kinds of genetic fingerprint by RAPD with the common primers, that is （1） V cholerae O139 and V cholerae O1 El Tor biotype, （2）V Cholerae O1 classical,and 3 kinds of genetic fingerprint with the repeative primers, that is （1）V cholerae O139,（2）V cholerae O1 El Tor biotype, （3）V Cholerae O1 classical The results of test was stable.Conclusion: It was simple, rapid, sensitive and accurate that PCR and RAPD were used to detect toxigenic V cholerae and identify their genetic pattern, which play a utilitary role in the clinical diagnosis,test of foods and the epidemiologic investigation