Effects of propofol on intestinal epithelial cells with oxidative stress injury

Objective:To study the effects of propofol on IEC in the oxidative stress injury induced by H₂O₂.Methods: IEC were cultured in intro,which were treated with H₂O₂,to simulate the IEC injured by OH from H₂O₂.IEC were divided into three group: control group,H₂O₂ group（2.5mmol/L） and propofol group（100μmol/L）.The IEC viability was observed by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT） assay.The lactatc dehydrogenase（LDH） that was relased into the culture media and the malondialdehyde（MDA） content in IEC were measured.Results: Compared with the control group,LDH in the culture media and MDA content in H₂O₂ group were significantly elevated,and the IEC viability obviously decreased（P＜0.01）.LDH in the culture media and MDA content in propofol group were lower than those of H₂O₂ group,and the IEC viability was obviously higher than that of H₂O₂ group.Conclusion: Propofol can protect rat IEC from the oxidative stress injury.