Cytotoxicity of chitosan thermosensitive hydrogel to human gingival fibroblasts in vitro

Objective To study the cytotoxicity of chitosan thermosensitive hydrogel to human gingival fibroblasts（HGF）for its application in the treatment of periodontal diseases. Methods Human gingival fibroblasts were cultured in a medium containing chitosan thermosensitive hydrogel at different concentrations for 24,48,72,and 96h,respectively.The relative growth rate（RGR）of HGF was determined by MTT assay. Results The relative growth rate of HGF was over 90%after cultured for different periods of time.The cytotoxicity grade of different concentrations of chitosan thermosensitive hydrogel was 0 and 1. Conclusion RGR have no cytotoxicity to HGF.Local application of chitosan thermosensitive hydrogel in the treatment of periodontal diseases is feasible.