Prepareation and identification of monoclonal antibody of alginate

WANG Rui; CHAI Dong; PEI Fei; FANG Yi; ZHU Man

WANG Rui, CHAI Dong, PEI Fei, FANG Yi, ZHU Man. Prepareation and identification of monoclonal antibody of alginateJ. ACADEMIC JOURNAL OF CHINESE PLA MEDICAL SCHOOL, 2004, 25(5): 353-354.

Citation:

WANG Rui, CHAI Dong, PEI Fei, FANG Yi, ZHU Man. Prepareation and identification of monoclonal antibody of alginateJ. ACADEMIC JOURNAL OF CHINESE PLA MEDICAL SCHOOL, 2004, 25(5): 353-354.

Citation:

WANG Rui, CHAI Dong, PEI Fei, FANG Yi, ZHU Man. Prepareation and identification of monoclonal antibody of alginateJ. ACADEMIC JOURNAL OF CHINESE PLA MEDICAL SCHOOL, 2004, 25(5): 353-354.

Prepareation and identification of monoclonal antibody of alginate

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Objective: To prepare and identify the hybridome clones of monoclonal antibody of alginate. Methods: The spleen cell was obtained from BALB/C by programmed immunization, and then was fused with myeloma cell line SP2/0. The positive clones were obtained by ELISA method. Antibody was detected by double agar gel immune diffusion test and ELISA. Results: Two kinds of hybridome clones of monoclonal antibody of alginate was obtained, which secrete IgM or IgG respectively.

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Prepareation and identification of monoclonal antibody of alginate

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