microRNA regulates KIR3DL1 expression in natural killer cells

Objective To study the potential mechanism of microRNA（miR）underlying the regulation of KIR3DL1 expression in human peripheral blood derived natural killer（NK） cells.Methods KIR3DL1-associated miR was searched from miR database using bioinformatics techniques.PGL3 plasmids containing KIR3DL13’UTR were constructed.PcDNA3.0 containing corresponding miR and KIR3DL13’UTR were co-transfected into 293T cells.miR that may regulate KIR3DL1 expression before and after mutation was detected by luciferase reporter assay.Results Ten miRs including miR-146b were searched from the Target Scan Database.After transfection of miR-146b,the activity of luciferase was decreased to 61.3%.After mutation of the KIR3DL1 3’UT,the activity of luciferase increased 91.4%.Conclusion miRNA 146b can specifically bind to KIR3DL13’UTR and participate in regulation of KIR3DL1 expression.