Effect of short hairpin RNA-targeted S100A4 on proliferation of breast cancer MCF-7 cells

Objective To study the effect of short hairpin RNA （shRNA）- targeted S100A4 on proliferation of breast cancer MCF-7 cells. Methods S100A4-shRNA expression vector, constructed and confirmed by sequencing, was transfected into MCF-7 cells using lipofectamine TM2000. Expression, proliferation and apoptosis of S100A4 in MCF-7 cells were detected by quantitative RT-PCR and Western blot 48h after transfection. S100A4-shRNA expression vector was transfected into breast cancer MCF-7 cells and G418 was selected and cloned. Proliferation of breast cancer MCF-7 cells in nude mice was detected by in vivo tumor formation test. Results S100A4-shRNA expression vector was constructed and transfected into MCF-7 cells as confirmed by sequencing. The constructed vector could effectively inhibit the expression of S100A4 in MCF-7 cells, decrease the proliferation of MCF-7 cells, and induce the cells into late apoptosis. No significant change in MCF-7 cells was observed after stable transfection of S100A4 mRNA expression vector. However, the volume and weight of tumor were significantly decreased compared with the control group. Conclusion S100A4-shRNA expression vector can significantly suppress S100A4 expression and inhibit the proliferation of MCF-7 cells.