The significance of endothelins, nitric oxide on the regulation of renal blood flow in burnt rats

Objective: To elucidate the significance of endothelins, nitric oxide on the regulation of renal blood flow in burnt rats. Methods: By use of a 30% TBSA (total body surface araa) Ⅲ degree burnt rat model, we detected the dynamic changes of the contents of endothelins and nitric oxide in postburn plasma and kidney. Meanwhile, the effects of endothelin receptor antagonists and NO donor on renal blood flow in burnt rats were observed. Results: The renal blood flow decreased sharply in burnt rats, accompanied by the increases of endothelins and nitric oxide in plasma and kidney. The ratio of ET/NO in kidney elevated significantly. The changes of ET and the ratio of ET/NO were significantly correlated with renal blood flow. Administration of endothelin receptor antagonists (PD₁₄₇₉₅₃, PD₁₄₅₀₆₅) and NO donor (SIN-1) was demonstrated to be able to reverse the decrease of blood flow in kidney postburn. Furthermore, better outcome could be achieved by blocking both endothelin receptors than by interfering with only ETA, and even much better result could be anticipated if NO donor was given together.Conclusion: The pathological alterations of endothelins, nitric oxide contributed to the regulation of renal blood flow postburn.