Influence of valsartan on PAPP-A,IGF-1 gene expression in HUVSMC

Objective: To study the effect of Valsartan on the expressions of the PAPP-A,IGF-1 in human umbilical vein smooth muscle cell. Methods: Under the condition of ox-LDL of 10^-5mol/L,HUVSMC is cultured with AngⅡ,and with the combination of AngⅡand Valsartan. Compared with normal cultivation without ox-LDL,the expressions of PAPP-A,IGF-1 are assayed using RT-PCR and ELISA. Results: The expressions of PAPP-A and IGF-1 that AngⅡ induces are significantly higher than those in other groups.The Valsartan group makes no significant differences compared with the ox-LDL group in the mRNA expressions of PAPP-A and IGF-1.The ex-cell expression of PAPP-A that Valsartan induces has significant difference compared with ones in other groups. Conclusion: AngⅡ induces the expressions of PAPP-A,IGF-1 while Valsartan can deregulate the role that AngⅡ plays,especially through the ex-cell pathway.