Rapid construction and identification of a cDNA library from human gastric cancer compound tissues

Objective: To construct of a lambda phage based cDNA library made from human gastric cancer compound tissue,and to screen and clong gastric cancer related genes.Methods: Total RNA and mRNA was extracted and converted to blunt ended,double stranded cDNA by Oligo（dT） mediated reverse transciption followed by adapter addition Column chromotography isolates residual adapters and small bands Ligation of cDNA to the vector and in vitro packaging of ligated cDNA and introduction into E coliY1090 Titer and amplify the phage.Results: The cDNA library has 3 2×10 ⁶ recombinants,the recombining ratio is 96% The titer of amplified library is 1 2×10 ¹² pfu/L-.Conclusion: The results indicacate that we constructed the human gastric cancer compound tissue cDNA library successfully