Method evaluation on plasma concentration of Sirolimous by MEIA

Objective: For evaluating reliability of Sirolimous assay reagent,chemical characters were determined.Methods: The precision,linearity and recovery rate and correlation of this method were evaluated.Results: The within-run CV of low,middle and high level were 2.4%,6.5%,1.8% respectively.The between-run CV of low,middle and high level were 8%,10.33%,11.9% respectively.In the range of 1.5-30 ng/ml,result had good linearity.The correlation coefficient of this method with HPLC was 0.9361.Conclusion: The method is simple,quick,accurate and suitable for monitoring the Sirolimous concentration of renal transplant patients.