Expression of osteocalcin and collagen Ⅰ in vitro model of vascular calcification

Objective：To detect the expression of osteocalcin（OC）and collagenⅠ（ColⅠ）in vitro model of vascular Calcification. Methods：1.Establish a model of vascular calcification in vitro：BASMCs were obtained by a modification of the explant method. Cells of passage 2-8 were cultured in standard medium for 4 days and then switched to calcification medium（containing 10mmo/l Lβ-glycerophosphate ［β-GP］，10^-7 mo/l L insulin and 50μg/L Vit C）for 10 days.The medium was replaced with fresh medium every 2 days；2.Calcium deposition in the cellular layer、OC in cultured supernatant and the expression of OC、ColⅠmRNA in calcified BASMCs.was analyzed. Results：β-GP induced extensive calcium deposition of BASMCs in a dose-dependent and time-dependent manner accompanying increased phosphorus content and decreased calcium content in cultured supernatant；OC in cultured supernatant was increased in a timedependent manner in calcified BASMCs，the highest amount appeared in 8th day and decreased slightly in 10th day；the expression of OC and Col Ⅰ mRNA in calcified BASMCs was increased by 128.05% and 116.84% repectively compared with those of control group.Conclusions： The expression of OC and Col Ⅰ was increased in calcified BASMCs，which shows have a high concordance with cardiovascular ectopic calcification.