The changes of tumor necrosis factor and interleukin 6 in lymph of hemorrhagic shock rats

Objective: The aim of the study was to measure tumor necrosis factor （TNF） and interleukin 6 （IL-6） in lymph of hemorrhagic shock rats and the effect of anisodamine on TNF and IL-6 production. Methods: The rat model of hemorrhagic shock was made by bloodletting from common carotid artery. The lymph were collected by intubating into chylocyst. TNF and IL-6 in lymph were determined by means of radioimmunoassay. Results: Hemorrhagic shock led to an increase of TNF and IL-6 in lymph. TNF peaked at 1 hour after hemorrhage （P＜0.001）,which was 1.71 times higher than that of prehemorrhage. IL-6 peaked at 1~2 hour after hemorrhage （P＜0.01）. Rats were transfused with all of the lost blood and administered with anisodamine. The levels of TNF and IL-6 were reduced but not significant （P ＞ 0.05）. Conclusion: The results indicate that the increase of TNF and IL-6 in lymph may be one of important factors to induce lymph circulatory failure after hemorrhagic shock, and anisodamine seems to be able to inhibit TNF and IL-6 production.