Setup model of screening aldose reductase inhibitor

Objective: To set up model of screening aldose reductase inhibitor.Methods: Aldose reductase was purified from rat lens.First,the activity of aldose reductase was assayed spectrophotometrically by following decrease in the absorption at 340nm over 3 min period with DL-glyceraldehydethe,10 mmol/L as substrate and NADPH,0.15 mmol/L as coenzyme.Then,we changed the condition respectively and independently.Last,the inhibition of aldose reductase from rat lens was compared by quercetin with rutoside by this model.Results: Each 1 ml cuvette contained: DL-glyceraldehydethe,10 mmol/L as substrate and NADPH,0.15mmol/L as coenzyme,the extract of aldose reductase,100 μl.The activity of aldose reductase was assayed at 340nm over 3 min period at 37 ℃.The inhibition of aldose reductase from rat lens by quercetin was better than rutoside from IR50 quercetin ＜ IR50 rutoside.Conclusion: This model is reasonable and convenient.