Screening of breast cancer stem cells model

objective: To study proporation of breast cancer stem cells in MCF7、 SKBR3 and 293 cells in order to provide a good cell model for clinical treating breast cancer and developing target drugs.Methods: To measure the expression of CD44 and CD24 in MCF7、 SKBR3 and 293 cells by flow cytometry.And to study the expression change of CD44 and CD24 after chemotherapy.Results: The expression proporation of breast cancer stem cells in MCF7、 SKBR3 and 293 cells was 0.20%±0.10%,1.37%±0.20% and 36.27%±3.44%,respectively.The expression proporation was increased from 36.27%±3.44% to 41.32%±1.06% in 293 cells after chemotherapy,and there were no obvious changes in MCF7 and SKBR3 cells.Conclusion: Breast cancer stem cells exist in MCF7、 SKBR3 and 293 cells.And 293 cells is good as breast cancer stem cells model.