Inhibitory effect of magnesium isoglycyrrhizinate on proliferation of acute lymphoblastic leukemia Molt4 cell line

Objective To observe the effect of magnesium isoglycyrrhizinate on proliferation of Molt4 cells. Methods Magnesium isoglycyrrhizinate was diluted to 8 groups at different concentrations（10g/L,1g/L,10^-1g/L,10^-2g/L,10^-3g/L,10^-4g/L,10^-5g/L,10^-6g/L）.The Molt4 cells were incubated with magnesium isoglycyrrhizinate solution at these concentrations,and cell growth status was chronologically observed under microscope.CCK-8 assay was employed to detect the ability of cells to proliferate and growth curve was plotted for calculating the inhibition ratio and 50% inhibiting concentration（IC₅₀）. Results Maganesium isoglycyrrhizinate inhibited the proliferation of Molt4 cells proliferation in a time-and dose-dependent manner.The proliferation of Molt4 cells was significantly inhibited on day 3 after treated with magnesium isoglycyrrhizinate at the concentration of 10^-3g/L（P＜0.05）.The inhibitory rate for 10g/L,1g/L,10^-1g/L,10^-2g/L and 10^-3g/L was 87.1%,64.6%,48.7%,47.5% and 31.7%,respectively.The IC₅₀ was about 0.013g/L. Conclusion Magnesium isoglycyrrhizinate can inhibit the proliferation of Molt4 cells,,thus providing an experimental basis for the treatment of hepatic lesion correlated with leukemia chemotherapy with magnesium isoglycyrrhizinate.