Effect of oxidative low density lipoprotein on placental growth factor acting on release of nitric oxide and endothelial cell adhesion molecules from human umbilical vein endothelial cells

Objective: To Study the effect of oxidative low density lipoprotein（ox-LDL）on placental growth factor 1（PLGF 1）acting on release of nitric oxide（NO）and endothelial cell adhesion molecules from human umbilical vein endothelial cells（ECV 304）. Methods: ECV-304（2×105）were incubated in a RPMI 1640 medium containing 2% heat-inactivated fetal bovine serum with PLGF-1（20,40,80ng/mL）in the absence or presence of ox-LDL.After the cells were cultured for 3,6,12 and 24h,the medium was collected for further use.Levels of soluble inter-cell adhesion molecule 1（sICAM-1）and soluble vascular cell adhesion molecule-1（sVCAM-1）in the supernatant were measured with an ELISA quality kit and expression of NO was analyzed according to its manufacturer’s instructions. Results: PLGF-1 induced secretion of NO and endothelial cell adhesion molecules in a dose-and time-dependent manner,while ox-LDL decreased the NO level.The expression of VCAM-1 was 5-6 folds higher than that in the control group.However,the expression of ICAM-1 was only 2-3 folds higher than that in the control group. Conclusion: Oxidative injury is an independent risk factor for atherosclerosis.PLGF-1 can induce endothelial activation and up-regulate the expression of endothelial adhesion molecules,thus further deteriorating diseases.Inflammatory reaction can be controlled by inhibiting the PLGF-1 bioactivity.