The effect of oxidized low density lipoprotein on rabbit vascular smooth muscle cell biological function and the pharmacological mechanism of atorvastatin

Objective: To investigate the effect of oxidized low density lipoprotein（oxLDL） on cytokins secretion of vascular smooth muscle cell （VSMC） and the intervention action of HMG-CoA reductase inhibitor,atorvastatin. Methods: Rabbit aortic VSMC was cultured in 6 groups. The level of tumor necrosis factore alpha（TNFα）,interleukin-6（IL-6） and interleukin-8（IL-8） were evaluated using ELISA method. Results: oxLDL increased the secretion of TNFα,IL-6 and IL-8 by 1.2～1.4-fold within 1 hour, reached to peak of 6.4～8.5-fold and sustained as high as 1.26～5.05-fold at 24 hours. In a concentration range of 0.05～1μmol/L,atorvastatin decreased the cytockins secretion in a concentration dependent manner, which could be abolished by cholesterol precurssor mevalonate（MVA）. Atorvastatin can reverse the above effects of oxLDL. Conclusions: oxLDL increase secretion of TNFα,IL-6 and IL-8. Atorvastatin can inhibit cytokins secretion stimulated with or without oxLDL by impeding the synthesis of MVA.