miRNA-based RNAi mitochondria-related genes TFAM and POLG

Objective To provide the data for mitochondria and stem cells by studying lentivirus-mediated RNAi mitochondria-related genes TFAM and POLG. Methods Mitochondria-related genes TFAM or POLG were selected.Three shRNAs were designed to construct RNAi lentivirus vectors based on miRNA.Lentivirus plasmids were packaged into 293FT cells,transfected into primary fibroblasts,and efficiency of RNAi was detected by quantitative PCR and Western blot,respectively. Results POLG-shRNA-1 decreased the TFAM and POLG expression of 75% and 85%,respectively. Conclusion Lentivirus-mediated RNAi vector can interfere the expression of miRNA-based POLG and TFAM.