The detection of coronavirus nucleic acid and construction of its gene profile in blood、feces、sputum and urine specimens from SARS patient

Objective:To detect the coronavirus‘s nucleic acid in blood、feces、sputum and urine specimens from SARS patient and construt the corresponding gene profile of coronavirus in those specimen using whole genomic gene chip of coronavirus. Methods:Total RNAs of coronavirus，which come from blood，feces，sputum and urine of SARS patients，were extracted，reverse transcripted using random primers and labeled to be probes by fluorescent dye through PCR reaction respectively.Then the four groups probes were hybridized with the whole genomic gene chip of coronavirus.Simltaneitily，probes from cultured coronavirus and healthy blood were used to be positive and negative control.Results:the nucleic acid of coronavirus was detected in all the four specimens，but the nucleic acid was less integrated compare with the whole genomic sequence of the coronavirus ，and the gene profile of the coronavirus which come from feces，urine and sputum specimens was similar.In addation，the sequence that code the S1 protein was more continuous in some specimens compare with other sequences，those specimens include 2of 23blood samples，8 of 13sputum samples，48 of 51 samples and 2 of 2 urine samples.Conclusion:The coronavirus‘s nucleic acid in blood、feces、sputum and urine specimens of SARS patient can be detected using whole genomic gene chip of coronavirus. and the corresponding gene profile of coronavirus in those specimen can be constructed also.