Detection of multidrug resistance gene expression in spun, dripped and smeared acute leukemia cells by in situ hybridization

Objective: To compare the results of different sampling methods in detecting multidrug resistance （MDR1） gene expression in acute leukemia cells. Methods: The MDR1 gene expression in acute leukemia cells from 10 patients were detected by in situ hybridization after the cells were spun, dripped and smeared on the sheet gasses. Results: The MDR1 mRNA was revealed in the cells from 7 of 10 leukemia patients. It was found that the positive rates of MDR1 gene expression in the spun cells were similar as dripped cells, but higher than smeared cells. The morphologic features of spun and dripped cells were much better than smeared cells.Conclusion: All three sampling methods（spin, drip and smear） may be used to detect MDR1 gene expression in leukemia cells. And the method of cytospins seems to be better than the methods of cell drip and cells smear.