Study on the antioxidant properties and mechanism of pollen and needle extracts from pinus massoniana in lipophilic model system

Objective: Antioxidant capacity and relative mechanism of pollen and needle extracts from pinus massoniana were evaluated in lipophilic model system.Methods: Copper induced LDL oxidation and Rose Bengal system in vitro were used.Results: Pollen ethanol extract had perfect inhibitory effect on free redical chain reaction,delaying diene conjugation formation in isolated LDL particles from human blood.The needle ethanol extract had better protective effect for LDL oxidation than pollen.In Rose Bengal system,extracts both from pollen and needle are equivalent inhibitors,where MPPD operates by two distinct mechanisms to prevent lipid peroxidation i.e,radical scavenging and copper chelation,whereas MPND only operates via the first mechanism.Conclusion: Both pollen and need from pinus massoniana have good antioxidant capacity in lipophilic model systems,but the mechanism is not quite the same.